Neo Phuchane

Neo Phuchane

Molecular, Cell, and Developmental Biology @ UCLA, working at the intersection of bioengineering and healthcare

DATE DESCRIPTION
2026.02 – Present Engineering AI-Designed Biosensors for Neurodegeneration

Engineering AI-Designed Biosensors for Neurodegeneration @ The Jason Zhang Lab - UCLA

Developing novel proteins to detect and discover therapeutic targets for neurodegenerative diseases.

2025.08 – 2025.12 Building Genetic Scorecards for iPSC derived Hematopoietic Stem Cells

Building Genetic Scorecards for iPSC derived Hematopoietic Stem Cells @ The Mikkola Lab - UCLA

Cord blood is the gold standard for blood stem cell transplants, but is inherently limited in supply because of its source. To develop an off-the-shelf HSC, I worked with researchers at the forefront of iPSC-derived HSCs and built computational genetic scorecards that compared the maturation of iPSC-derived HSCs with mature and nascent fetal HSCs, performing scRNA-seq across 20+ datasets from fetal liver, bone marrow, and cord blood.

2023.06 – 2023.08 Engineering Plasmids with Mucin Protein to Modify Cell Adhesion
Plasmid
Mucin

Engineering Plasmids with Mucin Protein to Modify Cell Adhesion @ The Morsut Lab - USC

Mucin proteins are heavily glycosylated macromolecules that can modulate cell-cell and cell-matrix adhesion through their gel-forming properties and steric hindrance effects. I designed and engineered fluorescent bacterial plasmids encoding mucin proteins which were then transfected into mammalian cells.

2021.06 – 2021.08 Simulating Tissue Morphogenesis
Tissue Morphogenesis
Tissue Morphogenesis 2D

Simulating Tissue Morphogenesis @ The Morsut Lab

A critical challenge in tissue engineering is controlling the spatial organization and morphogenesis of multicellular structures. Using Python and CompuCell3D, I conducted in silico sensitivity analysis by systematically varying parameters including cell type conversion thresholds, cell motility, cell-cell adhesion strengths, and cell-to-medium adhesion coefficients. This analysis identified critical design modules for axial elongation and contributed to our bioRxiv preprint on programming mammalian cell aggregate elongation with synthetic gene circuits.

2021.10 – 2022.06 Developing Biomanufacturing Protocols

Developing Biomanufacturing Protocols @ ASPIRE Biomanufacturing

Recombinant Taq polymerase offers cost-effective alternatives to its commercially available variants, but requires optimization for reliable performance. To develop a scalable production system, I optimized expression and lysis protocols and benchmarked our crude extract against commercial standards. Additionally, I ran biochemical and metabolic assays to characterize a novel bacterial strain discovered during the optimization process.